Production and purification of the multifunctional enzyme horseradish peroxidase
نویسندگان
چکیده
منابع مشابه
Removal efficiency of Enzyme Horseradish Peroxidase in Removal of Tetracycline and Ciprofloxacin from Synthetic Wastewater
Background and purpose: So far, different methods have been used to remove residual antibiotics from aquatic environments. This study investigated the efficiency of enzyme horseradish peroxidase (HRP) in presence of hydrogen peroxide in removal of Tetracycline and Ciprofloxacin in a batch system. Materials and methods: In an experimental study on laboratory scale, the effects of contact time, ...
متن کاملGlyco-variant library of the versatile enzyme horseradish peroxidase
When the glycosylated plant enzyme horseradish peroxidase (HRP) is conjugated to specific antibodies, it presents a powerful tool for medical applications. The isolation and purification of this enzyme from plant is difficult and only gives low yields. However, HRP recombinantly produced in the yeast Pichia pastoris experiences hyperglycosylation, which impedes the use of this enzyme in medicin...
متن کاملAn analysis of horseradish peroxidase enzyme for effluent treatment
The present study explains computational methods to design thermostable horseradish peroxidase enzyme using the crystal structure available from Protein Data Bank (PDB ID: 6ATJ). Multiple mutations were introduced to the original enzyme and developed a model by using Modeler9.14. After designing the model functional effect was confirmed in terms of protein ligand binding by molecular docking us...
متن کاملProduction of the superoxide radical by horseradish peroxidase.
The reaction mixture contained, in a total volume of 1.00m1, enzyme, p-coumaric acid (2.5pmol), dihydroxyfumaric acid (30pmol), KH2P04 (8.3pmol) and sufficient KOH to adjust the pH to 6. Incubations were carried out for 0.5h at 25°C and caffeic acid production was assayed as described by Halliwell(l975). 100% corresponded to a rate of 60nmol of caffeic acid formed in 0.5h. Oxidation of dihydrox...
متن کاملHorseradish peroxidase: a modern view of a classic enzyme.
Horseradish peroxidase is an important heme-containing enzyme that has been studied for more than a century. In recent years new information has become available on the three-dimensional structure of the enzyme and its catalytic intermediates, mechanisms of catalysis and the function of specific amino acid residues. Site-directed mutagenesis and directed evolution techniques are now used routin...
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ژورنال
عنوان ژورنال: Pharmaceutical Bioprocessing
سال: 2013
ISSN: 2048-9145,2048-9153
DOI: 10.4155/pbp.13.23